Device

Part:BBa_J119388:Design

Designed by: Alex Duryee, Grace Li   Group: Eckdahl Lab   (2015-06-10)


rClone Tet Red: Device for GGA Cloning and Testing RBS elements and Riboswitches


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1008
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1180
    Illegal NgoMIV site found at 1548
    Illegal NgoMIV site found at 1708
    Illegal AgeI site found at 2655
    Illegal AgeI site found at 2767
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849
    Illegal BsaI.rc site found at 42


Design Notes

None.


Source

PCR of J119385 and J119384 and BbsI GGA.

References